pEGFP-N2 荧光表达载体
pEGFP-N2 荧光表达载体
品牌:Solarbio | 货号:P6440
- 别名 : pEGFPN2, pEGFP N2
- 储存条件 : -20℃
- 单位 : 支
商品货号: | 商品品牌: | 规格 | 基本售价: | 选择规格 |
---|---|---|---|---|
P6440-20ul | Solarbio | 20ul | 2800.00元 |
产品介绍
保存:-20° C
载体信息:
启动子: CMV
复制子: pUCori,f1ori
终止子: SV40poly(A)
载体别名:pEGFPN2, pEGFP N2
载体分类:哺乳细胞表达载体,荧光蛋白报告载体
载体大小:4737bp
载体标签:C-EGFP
原核抗性:卡那霉素(Kanamycin)
筛选标记:新霉素(Neomycin)
克隆菌株:DH5α
表达宿主:哺乳细胞
培养条件:37℃,LB培养基
5' 测序引物:CMV-F: 5'-CGCAAATGGGCGGTAGGCGTG-3'
3' 测序引物:EGFP-N: 5'-CGTCGCCGTCCAGCTCGACCAG-3'
载体简介:
pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher expression in mammalian cells. (Excitation maximum = 488 nm;emission maximum = 507 nm.) pEGFP-N2 encodes the GFPmut1 variant which contains the double-amino-acid substitution of Phe-64 to Leu and Ser-65 to Thr