Cisplatin

MCE 国际站：Cisplatin

产品活性：Cisplatin (CDDP) 是一种抗肿瘤的化疗剂，它与 DNA 交联引起癌细胞中 DNA 损伤。Cisplatin 可激活铁死亡 (ferroptosis) 并诱导自噬 (autophagy)。

研究领域：Cell Cycle/DNA Damage | Apoptosis | Autophagy | Immunology/Inflammation | Metabolic Enzyme/Protease

作用靶点：DNA Alkylator/Crosslinker | Ferroptosis | Autophagy | Pyroptosis | Lipoxygenase

In Vitro: Cisplatin (CDDP) causes apoptosis of HeLa cells in a dose-dependent manner, with a concentration of 30 μM Cisplatin resulting in death of greater than 90% of the cell population by 24 h of treatment. The kinetics of Cisplatin-induced apoptosis are examined using a 30 μM concentration. Cisplatin Activates the MEK/ERK Signaling Pathway, 20 and 30 μM Cisplatin, both of which results in significant apoptosis, leads to strong activation of ERK.
Cisplatin (50 μM) produces time-dependent apoptosis in renal proximal tubular cell (RPTCs), causing cell shrinkage, a 50-fold increase in caspase 3 activity, a 4-fold increase in phosphatidylserine externalization, and 5- and 15-fold increases in chromatin condensation and DNA hypoploidy, respectively.

In Vivo: In melanoma-bearing mice, Cisplatin (CDDP; 4 mg/kg B.W.) reduces the size and weight of the solid tumors, and HemoHIM supplementation with Cisplatin enhances the decrease of both the tumor size and weight.
Cisplatin administration results in significant increases in the kidney weight as a percentage of the total body weight, urine volume, serum creatinine, and blood urea nitrogenby about 132, 315, 797, and 556% in comparison with the control rats, respectively.

Induction of Acute Kidney Injury (AKI)

Background

The pathogenesis of Cisplatin-induced acute kidney injury (AKI) is complex and Oxidative stress, mitochondrial dysfunction, inflammation, and apoptosis are all participating in the progression of Cisplatin-induced AKI.Oxidative stress is a predominant mechanism of injury in cisplatin-induced AKI.

Specific Mmodeling Methods

Mice: C57BL/6 • male • 6-week-old (period: 2 weeks)
Administration: 5 mg/kg • ip • once daily for 2 weeks

Note

(1) Suggest using male mice as female mice are more resistant to renal injury.
(2) Mice can be deprived of food and water for 18 h prior to induction, and food and water are returned after administration.
(3) Cisplatin can be dissolved in sterile saline solution to prepare injection working solution while protecting from light.

Modeling Record

Molecular changes: Increased indicators: Serum creatinine (SCr) levels, blood urea nitrogen (BUN) levels, neutrophil gelatinase-associated lipocalin (NGAL), 3-nitrotyrosine.

Correlated Product(s): 84-B10 (HY-44307)

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