BioXcell BE0101小鼠PD-L1单克隆抗体

BioXcell InVivoMab anti-mouse PD-L1 (B7-H1) 10F.9G2单克隆抗体与小鼠PD-L1（也称为B7-H1或CD274）反应。PD-L1是属于Ig超家族的B7家族的I型跨膜蛋白，分子量为40kDa。PD-L1在T淋巴细胞、B淋巴细胞、NK细胞、树突状细胞以及IFNγ刺激的单核细胞、上皮细胞和内皮细胞上表达。PD-L1与CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和骨髓细胞上的受体PD-1结合。PD-L1与PD-1的结合导致抑制TCR介导的T细胞增殖和细胞因子产生。PD-L1被认为在肿瘤免疫逃避中起着重要作用。诱导的PD-L1表达在许多肿瘤中很常见，并导致肿瘤细胞对CD8 T细胞介导的裂解的抗性增加。在黑色素瘤的小鼠模型中，可以通过用阻断PD-L1和PD-1之间相互作用的抗体处理来暂时肿瘤生长。BioXcell InVivoMab anti-mouse PD-L1 (B7-H1) 10F.9G2抗体已被证明可以阻断PD-L1和PD-1之间以及PD-L1和B7-1之间的相互作用（CD80）。

(BioXcell BE0101小鼠PD-L1单克隆抗体实验图)

产品详情：

产品名称 InVivoMAb anti-mouse PD-L1 (B7-H1)

产品货号 BE0101

产品规格 1/5/25/50/100mg

反应种属 Mouse

克隆号 10F.9G2

同种型 Rat IgG2a, κ

免疫原 Mouse CD274

实验应用

in vivo PD-L1 blockade

Immunofluorescence

Immunohistochemistry (frozen)

Flow cytometry

Western blot

产品形式 PBS, pH 6.5,Contains no stabilizers or preservatives

纯度 >95%, Determined by SDS-PAGE

无菌处理 0.2 µm filtration

纯化方式 Protein G

RRID AB_10949073

分子量 150 kDa

保存条件 抗体原液保存在4°C，不能冷冻保存。

推荐同型对照

InVivoMAb rat IgG2b isotype control, anti-keyhole limpet hemocyanin（货号BE0090）

推荐抗体稀释液

InVivoPure pH 6.5 Dilution Buffer（货号IP0065）

该产品自上市已被多篇SCI文献引用，品质有保证，以下是部分已发表的文献引用：

应用 文章

体内PD-L1信号阻断

(in vivo PD-L1 blockade)

1. Grasselly, C., et al. (2018). 'The Antitumor Activity of Combinations of Cytotoxic Chemotherapy and Immune Checkpoint Inhibitors Is Model-Dependent' Front Immunol 9: 2100.

2. Stathopoulou, C., et al. (2018). 'PD-1 Inhibitory Receptor Downregulates Asparaginyl Endopeptidase and Maintains Foxp3 Transcription Factor Stability in Induced Regulatory T Cells' Immunity 49(2): 247-263 e247.

3. Jaworska, K., et al. (2015). 'Both PD-1 ligands protect the kidney from ischemia reperfusion injury' J Immunol 194(1): 325-333.

4. Kim, J., et al. (2015). 'Memory programming in CD8(+) T-cell differentiation is intrinsic and is not determined by CD4 help' Nat Commun 6: 7994.

5. Zander, R. A., et al. (2015). 'PD-1 Co-inhibitory and OX40 Co-stimulatory Crosstalk Regulates Helper T Cell Differentiation and Anti-Plasmodium Humoral Immunity' Cell Host Microbe 17(5): 628-641.

6. Tkachev, V., et al. (2015). 'Programmed death-1 controls T cell survival by regulating oxidative metabolism' J Immunol 194(12): 5789-5800.

7. Twyman-Saint Victor, C., et al. (2015). 'Radiation and dual checkpoint blockade activate non-redundant immune mechanisms in cancer' Nature 520(7547): 373-377.

体内PD-L1阻断，流式细胞术

(in vivo PD-L1 blockade, Flow Cytometry)

1. loulou, M., et al. (2016). 'Follicular regulatory T cells can be specific for the immunizing antigen and derive from naive T cells' Nat Commun 7: 10579.

2. Ngiow, S. F., et al. (2015). 'A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1' Cancer Res 75(18): 3800-3811.

3. Rutigliano, J. A., et al. (2014). 'Highly pathological influenza A virus infection is associated with augmented expression of PD-1 by functionally compromised virus-specific CD8+ T cells' J Virol 88(3): 1636-1651.

体内PD-L1阻断，免疫荧光

(in vivo PD-L1 blockade, Immunofluorescence)

1.Willimsky, G., et al. (2013). 'Virus-induced hepatocellular carcinomas cause antigen-specific local tolerance' J Clin Invest 123(3): 1032-1043.

免疫组织化学（冷冻），免疫荧光

(Immunohistochemistry (frozen), Immunofluorescence)

1.Riella, L. V., et al. (2011). 'Essential role of PDL1 expression on nonhematopoietic donor cells in acquired tolerance to vascularized cardiac allografts' Am J Transplant 11(4): 832-840.

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