采用SPEX GENO做前处理研磨--水稻研究
• Genomics 89 (2007) 532–540
ScienceDirect www.sciencedirect.com
www.elsevier.com/locate/ygeno
Method
An efficient method for producing an indexed, insertional-mutant library in rice
水稻插入突变体库的有效建立方法—康奈尔大学
在文中,将多个水稻叶片同时在SPEX Geno 2000高通量组织研磨仪内进行研磨,研磨后的样品直接进行DNA提取,可以一次获得大规模的DNA样本。由于采用了高通量研磨仪,可以达到高效建立基因文库的效果。
Chengkun He, Moul Dey, Zhihong Lin, Faping Duan, Fengling Li, Ray Wu
Department of Molecular Biology and Genetics, Cornell University, 316 Biotechnology Building, Ithaca, NY 14853, USA
Received 9 August 2006; accepted 8 November 2006
Available online 16 January 2007