透明质酸氨基葡糖苷酶2(HYAL2)重组蛋白

YB237Mu011
Hyaluronoglucosaminidase 2 (HYAL2)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 透明质酸氨基葡糖苷酶2(HYAL2)重组蛋白[ PROPERTIES ]
Residues: Leu23~Gly447 (Accession # O35632),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cell membrane;
Lipid-anchor, GPI-anchor. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 7.3
 透明质酸氨基葡糖苷酶2(HYAL2)重组蛋白Predicted Molecular Mass: 50.2kDa
Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
 透明质酸氨基葡糖苷酶2(HYAL2)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MG H HH HH HS GS EF-LK PTAP PI FTG RPF VVAW NVP TQE CAP R HKV P L D L RA F
DVKATPNEGF FNQNITTFYY DRLGLYPRFD AAGTSVHGGV PQNGSLCAHL PMLKESVERY
IQTQEPGG LA VI DWEEW RPV WV RNWQEK DV YRQSS RQ LVA SRHP DWPS DR
VMKQAQYE FE FA A R QFM L N T LRYV KAV RPQ HLW GFY L FP D CY N HDYVQ NW
ESYTGRCPDV EVARNDQLAW LWAESTALFP SVYLDETLAS SVHSRNFVSF RVREALRVAH
THHANHALPV YVFTRPTYTR GLTGLSQVDL ISTIGESAAL GSAGVIFWGD SEDASSMETC
QYLKNYLTQL LVPYIVNVSW ATQYCSWTQC HGHGRCVRRN PSANTFLHLN ASSFRLVPGH
TPSEPQLRPE GQLSEADLNY LQKHFRCQCY LGWGGEQCQR NYKGAAG
[ REFERENCES ]
1. Strobl B., et al. (1998) Genomics 53:214-219. 2. Rai S.K., et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98:4443-4448. 3. Chang N.-S. (2002) BMC Cell Biol. 3:8-8. 4. Duh F.M., et al. (2005) Retrovirology 2:59-59.