单酰甘油脂肪酶(MGL)重组蛋白

YB223Hu012
Recombinant Lipase, Monoacylglycerol (MGL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
单酰甘油脂肪酶(MGL)重组蛋白[ PROPERTIES ]
Residues: Met1~Pro303
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q99685
Host: E. coli
Subcellular Location: Endoplasmic reticulum
membrane, plasma membrane, synapse.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
单酰甘油脂肪酶(MGL)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 37.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
单酰甘油脂肪酶(MGL)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
MPEESSPRRT PQSIPYQDLP HLVNADGQYL FCRYWKPTGT PKALIFVSHG AGEHSGRYEE
LA RM LMG L D L LVFA H DHVG H GQS EGE RMVV SDF HVFV R DV LQ HV DSMQK D
YPGLPVFLLG HSMGGAIAIL TAAERPGHFA GMVLISPLVL ANPESATTFK VLAAKVLNLV
LPNLSLGPID SSVLSRNKTE VDIYNSDPLI CRAGLKVCFG IQLLNAVSRV ERALPKLTVP
FLLLQGSADR LCDSKGAYLL MELAKSQDKT LKIYEGAYHV LHKELPEVTN SVFHEINMWV
SQRTATAGTA SPP