17-6000-86
            
IPG-BUFFER, PH 4-7L
        
        

            
17-6000-87
            
IPG-BUFFER, PH 3-10L
        
        

            
17-6000-88
            
IPG-BUFFER, PH 3-10NL
        
        

            
17-6001-10
            
IMMOBILINE DRYSTRIP PH 4-7,7CM
        
        

            
17-6001-11
            
IMMOBILINE DRYSTRIP PH3-10,7CM
        
        

            
17-6001-12
            
IMMOBILINE DRYSTRIP PH 3-10NL,
        
        

            
17-6001-13
            
IMMOBILINE DRYSTRIP PH4-7, 13C
        
        

            
17-6001-14
            
IMMOBILINE DRYSTRIPPH3-10,13CM
        
        

            
17-6001-15
            
IMMOBILINE DRYSTRIP PH3-10NL,
        
        

            
17-6001-78
            
IPG BUFFER, PH 6-11
        
        

            
17-6001-88
            
IMMOBILINE DRYSTRIP PH 6-9,18C
        
        

            
17-6001-94
            
IMMOBILINE DRYSTRIP PH 6-11,7C
        
        

            
17-6001-95
            
IMMOBILINE DRYSTRIP PH 6-11,11
        
        

            
17-6001-96
            
IMMOBILINE DRYSTRIP PH 6-11,13
        
        

            
17-6001-97
            
IMMOBILINE DRYSTRIP PH 6-11,18
        
        

            
17-6002-02
            
IPG BUFFER, PH 3-5.6 NL
        
        

            
17-6002-04
            
IPG BUFFER, PH 4.5-5.5
        
        

            
17-6002-05
            
IPG BUFFER, PH 5.0-6.0
        
        

            
17-6002-06
            
IPG BUFFER, PH 5.3-6.5
        
    


IPG Buffers

    
For use with Immobiline™ DryStrip gels to improve protein solubility.
    
Using Immobiline™ DryStrip gels together with matching IPG Buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.
    
Improved IPG Buffer pH 3-10 and pH 3-10 NL offer improved resolution, sensitivity, and increased spot intensity as well as additional protein spots in high molecular weight and basic pH areas.
    
Eliminates background staining.

IPG Buffers
Technical Information


 

IPG Buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. IPG Buffer pH 3-10 and pH 3-10 NL have been improved with a new formula to increase resolution, sensitivity, and spot intensity.
 
Each IPG Buffer produces a more uniform conductivity along the Immobiline DryStrip during focusing, resulting in wider latitude in run times. IPG Buffers also eliminate high background staining. IPG Buffers are supplied in 1 ml aliquots of water. The buffer is diluted 50- or 200-fold in the rehydration solution, depending on the first-dimension system and pH range of the strip.
 


    

        

            
TECHNICAL SPECIFICATIONS
        
        

            
Content 
            
Specialized carrier ampholytes in aqueous solution 
        
        

            
Volume 
            
1 ml 
        
        

            
Storage 
            
4°C to 8°C 
        
        

            
Shelf life 
            
36 months 
        
    


 
 

The basic area of 2-D protein spot maps using Immobiline™ DryStrip pH 3-10 NL (18 cm) together with A) improved IPG Buffer pH 3-10 NL or B) the previous recipe for IPG Buffer pH 3-10 NL. The improved IPG Buffer formula results in additional spots, and some of the differences are indicated in the figure.
http://www.gelifesciences.com/aptrix/upp01077.nsf/Content/Products?OpenDocument&moduleid=165728