RFC2,复制激活因子C2抗体

产品编号HZ-11305R
英文名称RFC2
中文名称复制激活因子C2抗体
别 名Activator 1; Activator 1 subunit 2; Activator 140 kDa Subunit; MGC3665; OTTHUMP00000160645; OTTHUMP00000160646; replication factor C (activator 1) 2 40kDa; Replication factor C (activator 1) 2; Replication factor C 2; Replication Factor C 40 kDa subunit; Replication factor C subunit 2; replication factor c2; Replication Factor C40 kDa subunit; RF C 40 kDa subunit; RF-C 40 kDa subunit; RFC 2; RFC 40; RFC 40 kDa subunit; RFC2; RFC2_HUMAN; RFC40; A1 40 kDa subunit; A1 antibody A140; Activator 1 40 kDa Subunit.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞凋亡 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
RFC2,复制激活因子C2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RFC2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
RFC2,复制激活因子C2抗体PubMedPubMed
产品介绍background:
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function. The human RFC2 gene maps to chromosome 7q11.23 and encodes the RFC2 subunit. RFC2 has been associated with Williams-Beuren syndrome, which is a rare multi-system developmental disorder caused by the deletion of contiguous genes at 7q11.23.

Function:
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP.

Subunit:
Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA. RFC2 also interacts with PRKAR1A; the complex may be involved in cell survival.

Subcellular Location:
Nucleus.

RFC2,复制激活因子C2抗体DISEASE:
Note=RFC2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.

Similarity:
Belongs to the activator 1 small subunits family.

Database links:
UniProtKB/Swiss-Prot: P35250.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: mouse intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-RFC2 Polyclonal Antibody, Unconjugated(HZ-11305R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining