Configured with Ni-E

The A1 MP⁺/A1R MP⁺ multiphoton confocal microscopes provide faster and sharper imaging from deeper within living organisms, extending the boundaries of traditional research techniques in biological sciences. They are compatible with both upright and inverted microscopes, and provide optimum multiphoton imaging configurations for brain research, other neuroscience applications and in vivo imaging of living specimens.

• Visualization of intravital microcirculation
  Blood cells in blood vessels within a living organism were excited by a femtosecond pulsed IR laser with the A1R MP⁺' ultrahigh-speed resonant scanner, and their movements were simultaneously captured in three successive fluorescence images at 30 fps (30 msec), with three separate color channels.
  Three fluorescent probes are simultaneously excited and imaged–nucleus (blue), endothelium (green), and plasma (red). The long-wavelength ultrafast laser in combination with the ultrahigh-speed resonant scanner effectively reduces photodamage and makes time resolved multiphoton imaging of biomolecules possible.
• Image resolution: 512 x 512 pixels, Image acquisition speed: 30 fps, Objective: water immersion objective 60x
• Imaged with the cooperation of: Dr. Satoshi Nishimura, Department of Cardiovascular Medicine, the University of Tokyo, TSBMI, the University of Tokyo, PRESTO, Japan Science and Technology Agency

• In vivo image of deep areas of cerebral cortex of a mouse
  The cerebral cortex of an H-line 5-week-old mouse was studied with the open skull method. The entire shape of dendrites of pyramidal cells in layer V expressing EYFP were visualized from the bottom layer into a superficial layer. In addition, the fluorescence signal of white matter in deeper areas was also studied.
• Left) 3D reconstruction image
  Right) Z-stack images

  Top:

  dendrites located in superficial layers in the layer V pyramidal cells
  25 µm from the surface

  Middle:

  basal dendrites in the layer V pyramidal cells
  625 µm from the surface

  Bottom:

  fluorescence from white matter

• Excitation wavelength: 930 nm
  Objective: CFI75 Apo 25xW MP (NA 1.10 WD 2.0)
  Excitation wavelength: 930 nm
  Objective: CFI75 Apo 25xW MP (NA 1.10 WD 2.0)
• Photographed with the cooperation of:
  Dr. Tomomi Nemoto, Research Institute for Electronic Science, Hokkaido University
  Dr. Shigenori Nonaka, National Institute for Basic Biology
  Dr. Takeshi Imamura, Graduate School of Medicine, Ehime University

The newly developed gallium arsenide phosphide (GaAsP) NDD* has approximately twice the sensitivity of a standard NDD and allows clear imaging of deeper areas of in vivo mouse brain over 1.2mm. Its ability to acquire bright images enables faster imaging and higher quality Z-stack imaging. Its high sensitivity allows acquisition of fluorescent signals with less laser power, resulting in less photo damage to living specimens.

• *Compatible with FN1 fixed stage microscope

• Deep brain imaging in in vivo mouse
  In vivo imaging of an anesthetized YFP-H mouse (4-week-old) via open skull method. Visualization of the entire layer V pyramidal neurons and the deeper hippocampal neurons. Deep imaging achieved for 3-dimensional imaging of hippocampal dendrites over 1.1 mm into the brain.
• Captured with episcopic GaAsP NDD and CFI75 Apochromat 25xW MP objective lens (NA 1.10, WD 2.0 mm)
  Photographed with the cooperation of:
  Dr. Ryosuke Kawakami, Dr. Terumasa Hibi, Dr. Tomomi Nemoto, Research Institute for Electronic Science, Hokkaido University

When the multiphoton laser wavelength or group velocity dispersion pre-compensation is changed, the multiphoton laser beam positional pointing at the objective back aperture may also change, resulting in uneven intensity across the image, or a slight misalignment between the IR and visible laser light paths.
Verifying the IR laser beam pointing and setting the alignment has traditionally been difficult. Nikon's A1 MP⁺ series' auto laser alignment function, housed in the Incident Optical Unit for the multiphoton excitation light path, automatically maximizes IR laser alignments with a single click in NIS-Elements C.

A1R MP⁺ hybrid scanner

A1 MP⁺ equipped with a galvano (non-resonant) scanner enables high-resolution imaging of up to 4096 x 4096 pixels and high-speed acquisition of 10 fps (512 x 512 pixels). A1R MP⁺ is a hybrid scanning head that incorporates both a galvano scanner and an ultrahigh-speed resonant scanner. A1R MP⁺ allows imaging and photoactivation at ultrafast speeds at 420 fps (512 x 32 pixels) necessary for revealing cell dynamics and interaction.