LDHB抗体
Applications
In-house applications |
ELISA, WB, IHC, IF, FC, IP Application not tested? Please see our Guarantee. |
Published applications |
IF, WB |
Publications |
4 citations –view here |
Species specificity |
Human,Mouse,Rat; other species not tested. |
Published Species |
Human, mouse |
Images
Prev12Next
- mouse brain tissue were subjected to SDS PAGE followed by western blot with 14824-1-AP(LDHB antibody) at dilution of 1:2000
- Immunohistochemical of paraffin-embedded human prostate using 14824-1-AP(LDHB antibody) at dilution of 1:100 (under 10x lens)
- Immunohistochemical of paraffin-embedded human prostate using 14824-1-AP(LDHB antibody) at dilution of 1:100 (under 40x lens)
- Immunofluorescent analysis of Hela cells, using LDHB antibody 14824-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- IP Result of anti-LDHB (IP:14824-1-AP, 3ug; Detection:14824-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
- 1X10^6 HeLa cells were stained with 0.2ug LDHB antibody (14824-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|
Positive WB detected in |
Mouse brain tissue, DU 145 cells, PC-3 cells, rat brain tissue, rat liver tissue |
Positive IP detected in |
Mouse brain tissue |
Positive IHC detected in |
Human prostate tissue |
Positive IF detected in |
Hela cells |
Positive FC detected in |
HeLa cells |
Recommended dilution |
WB : 1:1000-1:10000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:20-1:200 |
LDHB抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source |
Rabbit |
Purification method |
Antigen affinity purification |
Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information LDHB抗体
Immunogen |
LDHB fusion protein ag6605 |
Full name |
Lactate dehydrogenase B |
Calculated molecular
weight |
36 kDa |
Observed molecular
weight |
35 kDa |
GenBank accession
number |
BC002362 |
Gene ID (NCBI) |
3945 |
Gene symbol |
LDHB |
Synonyms |
Lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5 |
Background
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR. LDHB抗体
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