过氧化物酶体增殖物激活受体α(PPARα)重组蛋白

YB934Ra012
Peroxisome Proliferator Activated Receptor Alpha (PPARa)
Organism: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白[ DESCRIPTION ]
Protein Names: Peroxisome Proliferator Activated
Receptor Alpha
Synonyms: PPARa, NR1C1, PPAR
Species: Human
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Nucleus.
[ PROPERTIES ]
Residues: Pro61~Ala170 (Accession # Q07869), with
N-terminal His-Tag.
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白Grade & Purity: >95%, 15kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl.
Endotoxin Level: <1.0 EU per 1µg (determined by
the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 13.5kDa
Predicted isoelectric point: 8.9
Human PPARa
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
过氧化物酶体增殖物激活受体α(PPARα)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- PGSDGSVITD TLSPASSPSS VTYPVVPGSV DESPSGALNI
ECRICGDKAS GYHYGVHACE GCKGFFRRTI RLKLVYDKCD RSCKIQKKNR NKCQYCRFHK
CLSVGMSHNA