谷胱甘肽S转移酶π1(GSTπ1)重组蛋白

YB090Ra012
Glutathione Glutathione Glutathione Glutathione S Transferase Transferase Transferase Transferase Pi (GSTp)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
1th Edition (Revised in February, 2012)
谷胱甘肽S转移酶π1(GSTπ1)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Glutathione S Transferase Pi (GSTp); GSTP1; GST-P1; DFN7;
FAEES3; GST3
Gene Names: GSTP1; FAEES3; GST3
Size: 100µg（400 µL,250 µg/mL）
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: Conjugation of reduced glutathione to a wide number of exogenous and
endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35
translocation to prevent neurodegeneration.
谷胱甘肽S转移酶π1(GSTπ1)重组蛋白Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm
Tissue Specificity: Specificity: Specificity: Specificity: Ubiquitous
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1-Gln210 (Accession # P09211), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 24.6kDa as determined by SDS-PAGE reducing conditions.
Form & Buffer: 20% glycerol, 2mM EDTA, 12mM Sodium phosphate,150mM Sodium
chloride, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 24.6 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
谷胱甘肽S转移酶π1(GSTπ1)重组蛋白increase the chelating ability of the tag to Ni-Sepharose during purification.
Protein Sequence:MGHHHHHHSGS-MPPYTVVYFPVRGRCAALRMLLA DQGQSWKEEVVTVETWQEGSLKAS
CLYGQLPKFQDGDLTLYQSNTILRHLGRTLGLYGKDQQEAALVDMVNDGVEDLRCKYISLIYTNYEAGKDDYVKA
LPGQLKPFETLLSQNQGGKTFIVGDQISFADYNLLDLLLIHEVLAPGC LDAFPLLSAY VGRLSARPKLKAFLASPE
YVNLPINGNGKQ
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Kano T., Sakai M., et.al. (1987) Cancer Res. 47:5626-5630. 2. Ali-Osman F., Akande O., et.al.(1997) J. Biol. Chem. 272:10004-10012. 3. Alin P., Mannervik B., et.al. (1985) FEBS Lett. 182:319-322. 4. Gevaert K., Goethals M.,et.al. (2003) Nat. Biotechnol. 21:566-569. 5. Heibeck T.H., Ding S.-J., et.al. (2009) J. Proteome Res. 8:3852-3861. 6. Burkard T.R., Planyavsky M., et.al. (2011) BMC Syst. Biol. 5:17-17.