泛素特异性肽酶14(USP14)重组蛋白

YB134Hu016
Recombinant Ubiquitin Specific Peptidase 14 (USP14)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
泛素特异性肽酶14(USP14)重组蛋白[ PROPERTIES ]
Residues: Met1~Gln494
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P54578
Host: E. coli
Subcellular Location: Cytoplasm, Cell
membrane, Peripheral membrane protein.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
泛素特异性肽酶14(USP14)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.2
Predicted Molecular Mass: 59.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
泛素特异性肽酶14(USP14)重组蛋白which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MP LY S V T V KW GK E K F E GV E L NT DE P PM V F K AQ L FA LT GV Q PA R Q K VMV K G
GTLKDDDWGN IKIKNGMTLL MMGSADALPE EPSAKTVFVE DMTEEQLASA MELPCGLTNL
GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ YITAALRDLF DSMDKTSSSI
PPIILLQFLH MAFPQFAEKG EQGQYLQQDA NECWIQMMRV LQQKLEAIED DSVKETDSSS
ASAATPSKKK SLIDQFFGVE FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL
KLRLQEEITK QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP
LMLDMYELCT PELQEKMVSF RSKFKDLEDK KVNQQPNTSD KKSSPQKEVK YEPFSFADDI
GSNNCGYYDL QAVLTHQGRS SSSGHYVSWV KRKQDEWIKF DDDKVSIVTP EDILRLSGGG
DWHIAYVLLY GPRRVEIMEE ESEQ