糜蛋白酶原B1(CTRB1)重组蛋白

YB213Hu013
Recombinant Chymotrypsinogen B1 (CTRB1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 糜蛋白酶原B1(CTRB1)重组蛋白[ PROPERTIES ]
Residues: Ile34~Ala261
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P17538
Host: E. coli
Subcellular Location: Secreted, extracellular
space.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 糜蛋白酶原B1(CTRB1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 27.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
 糜蛋白酶原B1(CTRB1)重组蛋白which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
IVNGEDA VPGSWPWQVS LQDKTGFHFC GGSLISEDWV VTAAHCGVRT SDVVVAGEFD
QGSDEENIQV LKIAKVFKNP KFSILTVNND ITLLKLATPA RFSQTVSAVC LPSADDDFPA
GTLCATTGWG KTKYNANKTP DKLQQAALPL LSNAECKKSW GRRITDVMIC AGASGVSSCM
GDSGGPLVCQ KDGAWTLVGI VSWGSDTCST SSPGVYARVT KLIPWVQKIL A