RNF133,环指蛋白133抗体

产品编号HZ-9253R
英文名称RNF133
中文名称环指蛋白133抗体
别 名E3 ubiquitin protein ligase RNF133; MGC27072; Ring finger protein 133; RNF 133; RN133_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat,
RNF133,环指蛋白133抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RNF133
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
RNF133,环指蛋白133抗体PubMedPubMed
产品介绍background:
RNF133 contains a RING finger domain, a motif known to be involved in protein-protein and protein-DNA interactions. This gene has no intron. RNF133 may have E3 ubiquitin-protein ligase activity.

Function:
Has E3 ubiquitin-protein ligase activity (By similarity).

Subcellular Location:
Endoplasmic reticulum membrane; Single pass membrane protein.

Post-translational modifications:
Auto-ubiquitinated (By similarity).

Similarity:
Contains 1 PA (protease associated) domain.
Contains 1 RING-type zinc finger.

Gene ID:
168433

Database links:
Entrez Gene: 168433 Human
GenBank: NP_631914.1 Human
SwissProt: Q8WVZ7 Human
Unigene: 126730 Human

RNF133,环指蛋白133抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RNF133) Polyclonal Antibody, Unconjugated (HZ-9253R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.