CNG3,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体

产品编号HZ-10772R
英文名称CNG3
中文名称视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体
别 名ACHM2; CCNC1; CCNCa; CCNCalpha; CNCG3; CNG 3; CNG-3; CNG channel alpha 3; CNG channel alpha-3; CNG-3; CNG3; CNGA3; Cnga3 cyclic nucleotide gated channel alpha 3; CNGA3_HUMAN; Cone photoreceptor cGMP gated channel; Cone photoreceptor cGMP gated channel alpha subunit; Cone photoreceptor cGMP gated channel subunit alpha; Cone photoreceptor cGMP-gated channel subunit alpha; Cyclic nucleotide gated cation channel alpha 3; Cyclic nucleotide gated channel alpha 3; Cyclic nucleotide gated channel olfactory 3; Cyclic nucleotide gated channel rod photoreceptor subunit alpha; Cyclic nucleotide-gated cation channel alpha-3; Cyclic nucleotide-gated channel alpha-3.
说 明 书0.1ml 0.2ml
研究领域
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Pig, Cow, Horse, Sheep, Guinea Pig,
CNG3,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量79kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CNG3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CNG3,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体PubMedPubMed
产品介绍background:
Cyclic nucleotide-gated (CNG) cation channels are heteromeric complexes made up of principal alpha and modulatory beta subunits. The alpha subunits consist of CNG1-3 and form functional cation channels by themselves. The beta subunits consist of CNG4-6 and, unlike the alpha subunits, do not form functional channels, but rather modify the properties of channels. CNG channels are essential components of olfactory and visual transduction. In olfactory neurons, CNG2, CNG4.3 and CNG5 form Ca2+ permeable channels, which open and depolarize the cell in response to cAMP. In rod photoreceptors, CNG1 and CNG4.1 combine to form Ca ion permeable channels, which give rise to a current in response to cGMP. CNG3 and CNG6 are expressed in cone receptors and may combine to form a native cGMP-activated channel. CNG channels have been implicated in other areas. CNG1 is also expressed in medium-sized and small-sized arteries, suggesting a role for CNG in the regulation of arterial blood pressure and of blood supply to different regions. CNG1, CNG4.1 and CNG4.2 have been detected in the rat pineal gland. CNG2, CNG4.3 and CNG5 are present in GT1 cell lines and may play a role in the secretion of gonadotropin-releasing hormone.

Function:
Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3 (By similarity). Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones.

Subunit:
Tetramer formed of two CNGA3 and two CNGB3 modulatory subunits.

Subcellular Location:
Membrane; Multi-pass membrane protein.

Tissue Specificity:
Prominently expressed in retina.

Post-translational modifications:
Defects in CNGA3 are the cause of achromatopsia type 2 (ACHM2) [MIM:216900]; also known as total colorblindness or rod monochromacy (RMCH2). ACHM2 is an autosomal recessive condition characterized by day blindness and photophobia. In ACHM2 patients the cones are defective and the subjects see better at night.

Similarity:
Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family.
CNGA3 subfamily.
Contains 1 cyclic nucleotide-binding domain.

Gene ID:
1261

CNG3,视锥感光细胞环磷酸鸟苷门控通道蛋白CNG3抗体Database links:
Entrez Gene: 1261 Human
Omim: 600053 Human
SwissProt: Q16281 Human
Unigene: 234785 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CNG3 Polyclonal Antibody, Unconjugated(HZ-10772R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining