Musashi 1,神经干细胞RNA结合蛋白Musashi1抗体

产品编号HZ-11201R
英文名称Musashi 1
中文名称神经干细胞RNA结合蛋白Musashi1抗体
别 名Musashi 1 / Msi1; Msi 1; Msi1; Msi-1; MSI1H_HUMAN; Musashi homolog 1; Musashi-1; Musashi1; RNA binding protein Musashi homolog 1; RNA-binding protein Musashi homolog 1.
说 明 书0.1ml 0.2ml
研究领域肿瘤 神经生物学 干细胞
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
Musashi 1,神经干细胞RNA结合蛋白Musashi1抗体产品应用IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Musashi 1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Musashi 1,神经干细胞RNA结合蛋白Musashi1抗体PubMedPubMed
产品介绍background:
Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. Msi1 is the mammalian homolog of Drosophila Musashi. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.

Function:
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.

Subcellular Location:
Cytoplasm. Nucleus.

Tissue Specificity:
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.

Musashi 1,神经干细胞RNA结合蛋白Musashi1抗体Similarity:
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains.

Gene ID:
4440

Database links:
Entrez Gene: 4440 Human
Entrez Gene: 17690 Mouse
Entrez Gene: 259272 Rat
Omim: 603328 Human
SwissProt: O43347 Human
SwissProt: Q61474 Mouse
SwissProt: Q8K3P4 Rat
Unigene: 158311 Human
Unigene: 5077 Mouse
Unigene: 162146 Rat

Musashi 1,神经干细胞RNA结合蛋白Musashi1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Musashi1是一种进化保守的RNA结合蛋白，在维持干细胞状态、分化和肿瘤发生方面起着重要作用。Musashi1 选择性地表达在神经前体细胞上,包括神经干细胞上。在神经系统外，Musashi1还是肠干细胞的选择性标记。这些组织干细胞或未成熟细胞Musashi1的表达，表明Musashi1在转录后基因调节阶段维持这些细胞未分化状态起重要作用。Musashi1过度表达通过依赖RBP2Jk的旁路激活Notch1，而Notch信号途径功能为诱导哺乳动物神经干细胞自我更新。通过musashi1-P-小鼠培养脑细胞的Musashi蛋白产物反义去除研究,发现这些基因在维持神经干细胞未分化状态起着重要的作用。 Musashi抑制m-Numb转录的分子机制尚待进一步研究。Musashi1有可能除转录调控外还参与其他调控途径。另外，Musashi1还表达在一些脑肿瘤的特殊类型(这些肿瘤可能起源自非成熟脑细胞)，并且表达水平和肿瘤的恶性程度及增殖能力相关。

产品图片

Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Musashi-1 Polyclonal Antibody, Unconjugated(HZ-11201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: mouse pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Musashi-1 Polyclonal Antibody, Unconjugated(HZ-11201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining