人肝癌定量PCR芯片试剂盒描述：

ScienCells GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Kit (GQH-HCC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatocellular carcinoma tumorigenesis. Hepatocellular carcinoma is the most common type of liver cancer. It tends to occur in livers damaged by chronic infections of hepatitis B and/or C, alcohol abuse, type II diabetes and cirrhosis.  Brief examples of how included genes may be grouped are shown below:

• Chronic hepatitis B virus and hepatitis C virus infection response: AKT1, NFKB1, SOCS3, TGFB1
• DNA damage response and apoptosis: CASP8, MSH2, BAX, BCL2, BIRC5, FAS, TP53
• Cell cycling: CCND1, CCND2, CDKN1A, CDKN2A, BIRC5, MYC, PTEN, RASSF1
• Major signaling pathways involved in HCC tumorigenesis
  • Wnt/β-catenin: AXIN1, CCND1, CCND2, CTNNB1, EP300, FZD7, LEF1, RHOA, SFRP2, SMAD4
  • VEGF: VEGFA, AKT1, KDR, PTGS2, PTK2, RAC1, NRAS
  • FGF: AKT1, BAX, NFKB1, RAC1
  • MAPK: EGF, FAS, GADD45B, PDGFRA, TGFB1, TGFBR2, RAC1
  • PI3K: BCL2, FLT1, CREB3L3, IRS1, ITGB1, PTK1, MCL1, PTEN, RELN
• Other genes with demonstrated implications in HCC development: IGF2R, PDGFRL, MET, GLUL, MTUS1, FGL1, PEG10, MAGEC2, STARD13, FAH, GPC3

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Hepatocellular Carcinoma qPCR Array Plate Layout*

(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development