山梨醇脱氢酶(SDH)重组蛋白

YB495Ra011
Recombinant Sorbitol Dehydrogenase (SDH)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 山梨醇脱氢酶(SDH)重组蛋白[ PROPERTIES ]
Residues: Ala4~Lys344
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q58D31
Host: E. coli
Subcellular Location: Mitochondrion
membrane, Peripheral membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
 山梨醇脱氢酶(SDH)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 7.7
Predicted Molecular Mass: 40.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 山梨醇脱氢酶(SDH)重组蛋白The sequence of the target protein is listed below.
AKPENLS LVVHGPGDLR LENYPIPEPG PNEVLLKMHS VGICGSDVHY WQHGRIGDFV
VKKPMVLGHE ASGTVVKVGS LVRHLQPGDR VAIEPGAPRE TDEFCKIGRY NLSPTIFFCA
TPPDDGNLCR FYKHNANFCY KLPDNVTFEE GALIEPLSVG IHACRRAGVT LGNKVLVCGA
GPIGLVSLLA AKAMGAAQVV VTDLSASRLS KAKEVGADFI LQISNESPQE IAKKVEGLLG
SKPEVTIECT GVETSIQAGI YATHSGGTLV LVGLGSEMTS VPLVHAATRE VDIKGVFRYC
NTWPMAISML ASKSVNVKPL VTHRFPLEKA LEAFETSKKG LGLK