MICAL1,酪氨酸单氧化酶微管相关蛋白MICAL抗体

产品编号HZ-11525R
英文名称MICAL1
中文名称酪氨酸单氧化酶微管相关蛋白MICAL抗体
别 名Alternative namesCasL interacting molecule; MICA1_HUMAN; MICAL 1; MICAL; MICAL1; microtubule associated monoxygenase, calponin and LIM domain containing 1 antibody Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; NICAL.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Horse, Rabbit, Sheep,
MICAL1,酪氨酸单氧化酶微管相关蛋白MICAL抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量119kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human MICAL1 (101-200aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
MICAL1,酪氨酸单氧化酶微管相关蛋白MICAL抗体PubMedPubMed
产品介绍background:
MICAL1 is a 1,067 amino acid protein that localizes to both the cytoplasm and the cytoskeleton and contains one LIM zinc-binding domain and one calponin-homology domain. Expressed in kidney, thymus, spleen, lung and testis, MICAL1 interacts with the SH3 domain of Cas-L and, via this interaction, is thought to function as a cytoskeletal regulator that connects Cas-L to intermediate filaments. MICAL1 also interacts with Rab 1B, plexin-A3 and Vimentin, further regulating cytoskeletal events and possibly playing a role in axonal repulsion. Three isoforms of MICAL1 exist due to alternative splicing events.

Function:
Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization (Probable). Acts as a cytoskeletal regulator that connects NEDD9 to intermediate filaments. Also acts as a negative regulator of apoptosis via its interaction with STK38 and STK38L; acts by antagonizing STK38 and STK38L activation by MST1/STK4.

Subunit:
Interacts with STK38 and STK38L (By similarity). Associates with the SH3 domain of NEDD9. Interacts with VIM and PLXNA3. Interacts with RAB1B.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton.

Tissue Specificity:
Expressed in the thymus, lung, spleen, kidney, testis and hematopoietic cells.

Similarity:
Belongs to the Mical family.
Contains 1 CH (calponin-homology) domain.
Contains 1 LIM zinc-binding domain.

MICAL1,酪氨酸单氧化酶微管相关蛋白MICAL抗体Database links:
UniProtKB/Swiss-Prot: Q8TDZ2.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.