基底细胞粘附分子(BCAM)重组蛋白

YB716Hu014
Recombinant Basal Cell Adhesion Molecule (BCAM)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
基底细胞粘附分子(BCAM)重组蛋白[ PROPERTIES ]
Residues: Glu32~His257
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P50895
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
基底细胞粘附分子(BCAM)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 28.7kDa
Accurate Molecular Mass: 27&32kDa as determined by SDS-PAGE reducing
conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
基底细胞粘附分子(BCAM)重组蛋白Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
EVRLSVPPL VEVMRGKSVI LDCTPTGTHD HYMLEWFLTD RSGARPRLAS AEMQGSELQV
TMHDTRGRSP PYQLDSQGRL VLAEAQVGDE RDYVCVVRAG AAGTAEATAR LNVFAKPEAT
EVSPNKGTLS VMEDSAQEIA TCNSRNGNPA PKITWYRNGQ RLEVPVEMNP EGYMTSRTVR
EASGLLSLTS TLYLRLRKDD RDASFHCAAH YSLPEGRHGR LDSPTFH