FITC标记的E3连接酶MMS21蛋白抗体
产品名称： FITC标记的E3连接酶MMS21蛋白抗体
英文名称： Anti-NSE2/MMS21/FITC
产品编号： HZ-9544R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-NSE2/MMS21/FITC Conjugated antibody
FITC标记的E3连接酶MMS21蛋白抗体
| 产品编号 | bs-9544R-FITC |
| 英文名称 | Anti-NSE2/MMS21/FITC |
| 中文名称 | FITC标记的E3连接酶MMS21蛋白抗体 |
| 别 名 | C8orf36; E3 SUMO-protein ligase NSE2; hMMS21; hNSE2; methyl methanesulfonate sensitivity gene 21; MMS21; MMS21 homolog; non-SMC element 2; Non-SMC element 2 homolog; Non-structural maintenance of chromosomes element 2 homolog; NSE2_HUMAN; NSMCE2. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 细胞凋亡 表观遗传学 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 28kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMS21/NSE2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Breaks in double stranded DNA often arise during DNA replication or as a result of exposure to DNA-damaging agents. Quick and accurate repair of these breaks is crucial for cell survival and genomic stability. Structural maintenance of chromosomes (SMC) family members form heterodimeric complexes that modulate sister chromatid cohesion and chromosome condensation during mitosis. SMC5 and SMC6 play a crucial role in DNA repair as they form a complex with six conserved nonSMC subunits, including a ubiquitin E3 ligase NSE1 and a SUMO ligase NSE2. Specifically, this complex is crucial for sister chromatid homologous recombination DNA repair and also for prevention of chromosomal rearrangements. The NSE1 protein contains a RING-like motif that promotes DNA repair functions of the SMC5/SMC6 complex and full deletion of NSE1 is lethal to cells. NSE2 stimulates sumoylation of SMC6 and the DNA repair protein TRAX. Depletion of the NSE2 protein by RNA interference leaves the cell vulnerable to DNA damage-induced apoptosis. Function: E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. Acts as a E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, and maybe the cohesin components RAD21 and STAG2. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair. Subunit: Component of the SMC5-SMC6 complex which consists at least of SMC5, SMC6, NSMCE2, NSMCE1, NSMCE4A or EID3 and NDNL2. Subcellular Location: Nucleus.Chromosome, telomere. Note=Localizes to PML nuclear bodies in ALT cell lines. Post-translational modifications: Sumoylated, possibly via autosumoylation. Similarity: Belongs to the NSE2 family. Contains 1 SP-RING-type zinc finger. Database links: Entrez Gene: 286053 Human SwissProt: Q96MF7 Human Unigene: 388297 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
双链DNA的断裂通常在DNA复制或暴露于DNA损伤剂的过程中出现。快速和准确的修复这些断裂是至关重要的细胞存活和基因组稳定性。染色体（SMC）家族成员的结构维持形成异源二聚体复合物，在有丝分裂过程中调节姐妹染色单体结合和染色体缩合。SMC5和SMC6在DNA修复中起着至关重要的作用，因为它们形成具有六个保守的非SMMC亚基的复合物，包括泛素-E3连接酶NSE1和SUMO连接酶NSE2。特别地，这种复合物对于姐妹染色单体同源重组DNA修复和防止染色体重排是至关重要的。NSE1蛋白含有环状的基序，促进SMC5/SMC6复合物的DNA修复功能，NSE1的完全缺失对细胞是致命的。NSE2刺激SMC6和DNA修复蛋白TRAX的SUMO化。RNA干扰耗尽NSE2蛋白使细胞容易受到DNA损伤诱导的细胞凋亡。