纤连蛋白(FN)重组蛋白

YB037Ra011
Recombinant Fibronectin (FN)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 纤连蛋白(FN)重组蛋白[ PROPERTIES ]
Residues: Gly313~Ser607 (Accession # P02751),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
 纤连蛋白(FN)重组蛋白Predicted Molecular Mass: 35.2kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF - GVVYSVGM QWLKTQGNKQ MLCTCLGNGV SCQETAVTQT
YG GNS NGEP C VLP F TY N G RT FY S C T TE G RQ DG H LW CS T TS NY EQ DQK YSF
 纤连蛋白(FN)重组蛋白CT D HTV LVQ T RGG NS NGA L C HFPF LY N NH N YT DC TSEG R R DNMKW CG T TQ
NY DA DQKFGF CPMAAHEEIC TTNEGVMY RI GDQWDKQHDM GHMMRCTCVG
NG R GEW T CI A YS Q L R D Q CI V DD I T Y N V N D T FH K R H E E G HM LN C T C F G Q G R
GRWKCDPVDQ CQDSETGTFY QIGDSWEKYV HGVRYQCYCY GRGIGEWHCQ PLQTYPS
[ REFERENCES ]
1. Gutman A., et al. (1986) FEBS Lett. 207:145-148. 2. Dean D.C., et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84:1876-1880. 3. Kornblihtt A.R., et al. (1985) EMBO J. 4:1755-1759. 4. Iida R., et al. (2007) FEBS J. 274:3939-3947.