His标签抗体
产品名称： His标签抗体
英文名称： His Tag Antibody
产品编号： ICP0850
产品价格： null
产品产地： 加拿大
品牌商标： ImmuneChem
更新时间： null
使用范围： ELISA WB IP IHC
南宁市蓝光生物技术有限公司
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- 地址 : 广西南宁市高新区科园大道68号东盟慧谷11栋203号
- 邮编 : 530007
- 所在区域 : 广西
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关于 His-Tag, Antibody 的详细介绍
Catalog # Product Description | ICP0850 This high affinity His-tag antibody is developed using a polyhistidine peptide (HHHHHH) conjugated to KLH and affinity-purified with the HHHHHH peptide on agarose. The antibody is useful for detection, affinity isolation, enrichment and localization of His-tag proteins. |  Western-blot with His-tag antibody, HRP (ICP0851) of the His-tag protein (50 kDa) expressed cell lysate and enriched His-tag protein by IP with anti-His-tag (ICP0850) 1: Molecular marker 2: 10 μL of His-tag protein in lysate (original cell lysate) 3: 10 μL of His-tag protein in lysate (after IP) 4: 10 μL of the His-tag protein in 100 μL of the IP eluting buffer, enriched with 25 μg of the anti-His tag (ICP0850) from 2 mL of the original lysate | |
Species | Rabbit | ||
Formulation | 250 μg/mL in PBS, 50% glycerol | ||
Immunogen | Polyhistidine peptide (HHHHHH) KLH conjugates | ||
Purification | Antigen-specific affinity chromatography | ||
Specificity | The antibody recognizes His-tag proteins. | ||
Applications | ELISA (0.25-1 μg/mL); IP ( 1-10 μg, based on the sample size) ; WB (0.25-1.0 μg/mL); IHC (5 μg/mL) | ||
Scientific Description | Polyhistidine peptide is frequently used as an affinity tag to facilitate the isolation of the 6H-fusion proteins using a metal-chelate matrix. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is three years from date of shipping if stored properly. |
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