来源
用途
交叉反应性
分子量
Rabbit
WB, FC, IHC, ICC, IF
H, M
20KDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig;Rb, Rabbit; S, Sheep; Z, Zebrafish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:500-1:1000
-
1:200
1:200
1:200
1:100
-
-
抗体详细信息如下:
About this Antibody
Name
PUMA Rabbit Polyclonal Antibody
Category
Polyclonal antibody(pAb); Primary antibody
Isotype
IgG
Purification
Peptide affinity purified
About the Immunogen
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to PUMA.
Gene ID
27113
SwissProt
Q9BXH1
Synonyms
BBC3; bcl2 binding component 3; jfy1; puma; p53 up-regulated modulator of apoptosis
Category
-
Background
The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis.
包装清单：
产品编号
产品名称
包装
AF0270
PUMA Rabbit Polyclonal Antibody
50μl
AZ050
Western一抗稀释液
50ml
—
说明书
1份
保存条件：
PUMA Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
代表性图片：
Fig. 1. Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.Positive control: Lane 1: Hela Lane 2: Mouse kidney.
Fig. 2. Immunocytochemistry staining PUMA in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100/PBS.
Fig. 3. Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PUMA antibody. Counter stained with hematoxylin.
Fig. 4. Flow cytometric analysis of Jurkat cells with PUMA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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