载脂蛋白A5(APOA5)重组蛋白

YB997Hu011
Apolipoprotein Apolipoprotein Apolipoprotein Apolipoprotein A5 (APOA5) (APOA5) (APOA5) (APOA5)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 载脂蛋白A5(APOA5)重组蛋白6th Edition (Revised in March, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Asp167~Lys335 (Accession # Q6Q788),
with N-terminal His-Tag.
Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
 载脂蛋白A5(APOA5)重组蛋白pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.7
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 20.4kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 载脂蛋白A5(APOA5)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- DEAW ALLQGLQSRV VHHTGRFKEL FHPYAESLVS GIGRHVQELH
RSVAPHAPAS PARLSRCVQV LSRKLTLKAK ALHARIQQNL DQLREELSRA FAGTGTEEGA
GPDPQMLSEE VRQRLQAFRQ DTYLQIAAFT RAIDQETEEV QQQLAPPPPG HSAFAPEFQQ
TDSGK
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Zhang X., et al. (2012) Am. J. Clin. Nutr. 96:917-922. 2. Zheng X.Y., et al. (2012) Biol. Chem. 393:161-167. 3. Pi Y., et al. (2012) Cerebrovasc. Dis. 33:558-565. 4. Ding Y., et al. (2012) J. Biomed. Biotechnol. 2012:509420-509420.