PARP16,多腺苷二磷酸多聚酶16抗体

产品编号HZ-9623R
英文名称PARP16
中文名称多腺苷二磷酸多聚酶16抗体
别 名C15orf30; Chromosome 15 open reading frame 30; EC 2.4.2.30; FLJ25281; PAR16_HUMAN; PARP 16; PARP-16; Parp16; Poly (ADP ribose) polymerase family member 16; Poly [ADP ribose] polymerase 16; Poly [ADP-ribose] polymerase 16.
说 明 书0.1ml 0.2ml
研究领域肿瘤 免疫学 神经生物学 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,
PARP16,多腺苷二磷酸多聚酶16抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞核 细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PARP16
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PARP16,多腺苷二磷酸多聚酶16抗体PubMedPubMed
产品介绍background:
Poly(ADP-ribosylation) is a method of DNA damage-dependent posttranslational modification that helps to rescue injured proliferating cells from cell death. The PARP (poly(ADP-ribose) polymerase) proteins comprise a superfamily of enzymes that functionally modify histones and other nuclear proteins, thereby preventing cell death. PARPs use NAD+ as a substrate to catalytically transfer ADP-ribose residues onto protein acceptors; a process that, when repeated multiple times, leads to the formation of poly(ADPribose) chains on the protein. The presence of these chains alters the function of the target protein and promotes cell survival. PARP proteins are implicated in a variety of diseases, including cancer, neurodegenerative and inflammatory disorders. PARP-16 is a 322 amino acid poly (ADP-ribose) polymerase protein localized to the membrane. Expressed as three isoforms produced by alternative splicing, PARP-16 contains one PARP catalytic domain.

Function:
Mono-ADP-ribosyltransferase targeting the karyopherin KPNB1. Plays a role in unfolded protein response (UPR), by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors.

Subcellular Location:
Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.

Post-translational modifications:
ADP-ribosylated (-auto).

Similarity:
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.

Gene ID:
54956

PARP16,多腺苷二磷酸多聚酶16抗体Database links:
Entrez Gene: 54956 Human
Entrez Gene: 214424 Mouse
Entrez Gene: 315760 Rat
SwissProt: Q8N5Y8 Human
SwissProt: Q7TMM8 Mouse
SwissProt: Q5U2Q4 Rat
Unigene: 30634 Human
Unigene: 31129 Mouse
Unigene: 23423 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP16) Polyclonal Antibody, Unconjugated (HZ-9623R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.