SEMA4F,跨膜蛋白SEMA4F抗体

产品编号HZ-11478R
英文名称SEMA4F
中文名称跨膜蛋白SEMA4F抗体
别 名M SEMA; m Sema M; PRO2353; Sema domain immunoglobulin domain (Ig) transmembrane; Sema M; Sema W; SEMAM; Semaphorin M; Semaphorin W; SEMAW; SEM4F_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 细胞分化
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse,
SEMA4F,跨膜蛋白SEMA4F抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量80kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human SEMA4F (239-288aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
SEMA4F,跨膜蛋白SEMA4F抗体PubMedPubMed
产品介绍background:
Semaphorins are a family of cell surface and secreted proteins that are conserved from insects to humans. Members of this family of proteins are approximately 750 amino acids in length (including signal sequences) and are defined by a conserved extracellular “semaphorin” domain of approximately 500 amino acids containing 14-16 cysteines, blocks of conserved sequences and no obvious repeats. Secreted and cell-bound semaphorins chemically attract and repel the growth of neural axons, guiding the development of intricate networks of neural tissue. SEMA4F (semaphorin-4F), also known as SEMAM, SEMAW or PRO2353, is a 770 amino acid member of the semaphorin family. Localized to the membrane, SEMA4F is a single-pass type I protein that is involved in growth cone collapse of retinal ganglion-cell axons. SEMA4F is highly expressed in postnatal brain and lung and contains one immunogloblin-like (Ig-like) domain, one PSI domain and one semaphorin domain. Two isoforms exist due to alternative splicing events.

Function:
SEMA4F has growth cone collapse activity against retinal ganglion-cell axons.

Subcellular Location:
Membrane; Single-pass type I membrane protein

Similarity:
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.

Database links:
UniProtKB/Swiss-Prot: O95754.2

SEMA4F,跨膜蛋白SEMA4F抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-SEMA4F Polyclonal Antibody, Unconjugated(HZ-11478R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining