神经元正五聚蛋白Ⅰ(NPTX1)重组蛋白

YB298Ra011
Neuronal Pentraxin I (NPTX1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 神经元正五聚蛋白Ⅰ(NPTX1)重组蛋白[ PROPERTIES ]
Residues: Asn146~Arg384 (Accession # Q15818),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasmic vesicle,
secretory vesicle.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
 神经元正五聚蛋白Ⅰ(NPTX1)重组蛋白Predicted isoelectric point: 6.0
Predicted Molecular Mass: 28.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 神经元正五聚蛋白Ⅰ(NPTX1)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- NLEQY SRLNSSSQTN SLKDLLQSKI DELERQVLSR VNTLEEGKGG
PRNDTEERVK IETALTSLHQ RISELEKGQK DNRPGDKFQL TFPLRTNYMY AKVKKSLPEM
YAFTVCMWLK SSATPGVGTP FSYAVPGQAN ELVLIEWGNN PMEILINDKV AKLPFVINDG
KW HHI CVTW T TRD GVWEAYQ DG TQ GGSGE N LAPY HPIKPQ GV LV LG QEQ D
TLGGGFDATQ AFVGELAHFN IWDR