CD45抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
CD45抗体

CD45抗体

商家询价

产品名称： CD45抗体

英文名称： CD45Antibody

产品编号： XY20103-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

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 CD45抗体

Applications
Tested Applications: ELISA, IHC, WB, FC, IP
Specificity Reaction: Human, Mouse; Other species are not tested.Please decide the specificity by homology
Positive WB detected in Mouse liver tissue
Positive IP detected in Mouse spleen tissue
Positive IHC detected in Human lymphoma tissue, human tonsil tissue, human tonsillitis tissue
Positive FC detected in Jurkat cells
Recommended dilution WB : 1:200-1:1000
IP : 1:200-1:1000
IHC : 1:20-1:200
FC : N/A
CD45抗体Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
Prev12NextView more validation data
  • 20103-1-AP;human lymphomaImmunohistochemical of paraffin-embedded human lymphoma using 20103-1-AP(CD45 antibody) at dilution of 1:50 (under 10x lens)
  • 20103-1-AP;human lymphomaImmunohistochemical of paraffin-embedded human lymphoma using 20103-1-AP(CD45 antibody) at dilution of 1:50 (under 40x lens)
  • 20103-1-AP;mouse liver tissuemouse liver tissue were subjected to SDS PAGE followed by western blot with 20103-1-AP( CD45 Antibody) at dilution of 1:300
  • 20103-1-AP;mouse spleen tissueIP Result of anti-CD45 (IP:20103-1-AP, 4ug; Detection:20103-1-AP 1:300) with mouse spleen tissue lysate 4800ug.
  • 20103-1-AP;Jurkat cells1X10^6 Jurkat cells were stained with 0.5ug CD45 antibody (20103-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Product Information
Source: Rabbit Purify: Antigen affinity purification
IsoType: IgG Storage: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. -20oC, Avoid freeze / thaw cycles.
Immunogen InformationCD45抗体
Immunogen: CD45 fusion protein ag13712 Full Name: Protein tyrosine phosphatase, receptor type, C
Caculated Molecular
Weight:		 1304aa,147 kDa Observed Molecular 
Weight:		 180-220 kDa
GenbankNo BC014239 GeneID: 5788
Symbol PTPRC Synonyms B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200
Background

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.CD45抗体

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