Tsukushin,TSK蛋白抗体

产品编号HZ-11607R
英文名称Tsukushin
中文名称TSK蛋白抗体
别 名E2 induced gene 4 protein; E2IG4; Leucine rich repeat containing protein 54; LRRC54; TSK; Tsukushi; TSK_HUMAN; Tsukushin.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 干细胞 生长因子和激素 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Horse, Rabbit,
Tsukushin,TSK蛋白抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位分泌型蛋白
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Tsukushin
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Tsukushin,TSK蛋白抗体PubMedPubMed
产品介绍background:
The leucine-rich (LRR) repeat is a 20-30 amino acid motif that forms a hydrophobic å/∫ horseshoe fold, allowing it to accommodate several leucine residues within a tightly packed core. All LRR repeats contain a variable segment and a highly conserved segment, the latter of which accounts for 11 or 12 residues of the entire LRR motif. LRRC54 (leucine-rich repeat-containing protein 54), also known as tsukushin, TSKU or E2-induced gene 4 protein (E2IG4), is a 353 amino acid secreted protein that likely localizes to the cell membrane and extracellular compartments. Involved in extracellular secretion and intracellular transport, LRRC54 can be induced by 17-beta-estradiol. Containing nine LRR repeat and a cleavable signal peptide, the gene encoding LRRC54 maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.

Function:
It is suggested that Tsukushin is involved in intracellular transport and extracellular secretion. Its structure contains 9 LRR (leucine-rich) repeats.

Subcellular Location:
Secreted

Similarity:
Contains 10 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.

Gene ID:
25987

Database links:
Entrez Gene: 25987 Human
GenBank: NM_015516 Human
GenBank: NP_056331 Human
Omim: 608015 Human
SwissProt: Q8WUA8 Human
Unigene: 8361 Human

Tsukushin,TSK蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti- Tsukushin Polyclonal Antibody, Unconjugated(HZ-11607R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining