上皮型脂肪酸结合蛋白(FABP5)重组蛋白

YB985Ra011
Fatty Acid Binding Binding Binding Binding Protein Protein Protein Protein 5, Epidermal Epidermal Epidermal Epidermal (FABP5) (FABP5) (FABP5) (FABP5)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
1th Edition (Revised in February, 2012)
 上皮型脂肪酸结合蛋白(FABP5)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Fatty Acid Binding Protein 5, Epidermal
Gene Names: FABP5
Size: 100µg
Source: Recombinant
Expression Expression Expression Expression Host: E.coli
Function: Function: Function: Function: High specificity for fatty acids. Highest affinity for C18 chain length.
Decreasing the chain length or introducing double bonds reduces the affinity. May
 上皮型脂肪酸结合蛋白(FABP5)重组蛋白be involved in keratinocyte differentiation.
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm.
Tissue Specificity: Specificity: Specificity: Specificity: Keratinocytes; highly expressed in psoriatic skin.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Ala2~Glu135 (Accession # Q01469), with a N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >97%, 16.3 kDa as determined by SDS-PAGE reducing
conditions.
Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 16.3 kDa
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS.
 上皮型脂肪酸结合蛋白(FABP5)重组蛋白[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term
storage. Avoid repeated freeze/thaw cycles.
Valid period: period: period: period: 12 months stored at -80oC.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with a His-tag and its sequence is listed below. The first Met is an initiator amino acid. Moreover, Gly and Ser are added to improve the flexibility of N-terminus at both ends of the His-tag, which will
increase the chelating ability of the tag to Ni-Sepharose during purification.
MGHHHHHHSGS-ATVQQLEGR WRLVDSKGFD EYMKELGVGI ALRKMGAMAK PDCIITCDGK NLTIKTESTL
KTTQFSCTLG EKFEETTADG RKTQTVCNFT DGALVQHQEW DGKESTITRK LKDGKLVVEC VMNNVTCTRI
YEKVE
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Madsen P.S., et al. (1992) J. Invest. Dermatol. 99:299-305.
2. Gutierrez-Gonzalez L.H., et al. (2002) Biochem. J. 364:725-737.
3. Hohoff C., et al. (1999) Biochemistry 38:12229-12239.