C16ORF84抗体
Applications
| In-house applications |
ELISA, WB, IHC, IF, IP Application not tested? Please see our Guarantee. |
| Species specificity |
Human, Mouse; other species not tested. |
|
Images
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 HepG2 cells were subjected to SDS PAGE followed by western blot with 23337-1-AP( C16orf84 Antibody) at dilution of 1:600 IP Result of anti-C16orf84 (IP:23337-1-AP, 4ug; Detection:23337-1-AP 1:500) with HepG2 cells lysate 3600ug. Immunohistochemistry of paraffin-embedded human testis tissue slide using 23337-1-AP( C16orf84 Antibody) at dilution of 1:50 (under 40x lens) Immunohistochemistry of paraffin-embedded human kidney tissue slide using 23337-1-AP( C16orf84 Antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of HEK-293 cells using 23337-1-AP( C16orf84 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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| Positive WB detected in |
HepG2 cells, mouse liver tissue, mouse spleen tissue, Raji cells |
| Positive IP detected in |
HepG2 cells |
| Positive IHC detected in |
Human testis tissue, human kidney tissue |
| Positive IF detected in |
HEK-293 cells |
| Recommended dilution |
WB : 1:200-1:2000
IP : 1:200-1:2000
IHC : 1:20-1:200
IF : 1:20-1:200 |
C16ORF84抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
C16orf84 fusion protein ag18428 |
Full name |
Chromosome 16 open reading frame 84 |
Calculated molecular
weight |
515aa,56 kDa |
Observed molecular
weight |
65-68 kDa |
GenBank accession
number |
BC125269 |
Gene ID (NCBI) |
348180 |
| Gene symbol |
C16orf84 |
Synonyms |
C16orf84, CTU2, NCS2, UPF0432 |
Background C16ORF84抗体
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Cytoplasmic tRNA 2-thiolation protein 2 (CUT2), also named as C16orf84 or NCS2, is a 515 amino acid protein, which belongs to the CUT2 family. C16orf84 localizes in the cytoplasm and Plays a central role in 2-thiolation of mcm5S2U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). C16orf84 may act by forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from thio-carboxylated URM1 onto the uridine of tRNAs at wobble position. C16ORF84抗体
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