Monoclonal Antibody against Human Cytokeratin, clone AE1/AE3
产品名称： Monoclonal Antibody against Human Cytokeratin, clone AE1/AE3
英文名称： Monoclonal Antibody against Human Cytokeratin, clone AE1/AE3
产品编号： MMS-137P
产品价格： null
产品产地： USA
品牌商标： COVANCE
更新时间： 2023-08-17T13:40:59
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
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- 邮箱 : lizizhuan@yeasen.com
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| Description |  |
Monoclonal Antibody against Human Cytokeratin, clone AE1/AE3 |
| Intended Use | |
**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | |
AE1/AE3 |
| Form | |
Affinity Purified Antibody (0.05 M Tris-HCl, pH 7.2, + 0.015 mol/L sodium azide) |
| Host | |
Mouse |
| Species Reactivity | |
Human |
| IsoType | |
IgG1 |
| Specificity | |
Cytokeratins are a family of water-soluble proteins with molecular weights between 40-70 kD that form the cytoskeleton of epithelial cells. At least 19 different cytokeratins have been identified and can be divided into two subfamilies. Subfamily A comprises relatively acidic cytokeratins (with a pI under 5.5) whereas members of subfamily B have a relatively basic pI of 6 or over. AE1/AE3 is a cocktail of two monoclonal antibodies that were obtained by immunizing mice with human callus keratins. AE1/AE3 has been shown to identify the majority of human cytokeratins and thus may be used as a tool for the positive IHC identification of cells of simple and stratified epithelial origin. Antibody AE1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with Moll's designation 4, 10, 13, 14, 15 16 and 19 (MWs of 56.5, 54', 50, 50', 48 and 40 kD,respectively) but not on Nos. 12, 17 and 18 (55, 47 and 45 kD). Antibody AE3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including Nos. 1 and 2, 3, 4, 5, 6, 7 and 8 (MWs of 65, 67, 64, 59, 58, 56, 54 and 52 kD, respectively). |
| Uses | |
AE1/AE3 can be used on formalin-fixed, paraffin-embedded tissue sections and for labelling acetone-fixed cryostat sections or fixed cell smears. The cellular staining pattern for AE1/AE3 is cytoplasmic. |
| Suggested Working Dilution | |
The optimal working dilution should be determined for each specific assay condition.
|
| Notes | |
*Tissue pretreatment, by proteolytic enzymes or heat-induced epitope retrieval for epitope enhancement is required. The following enzymes can be used for pretreatment of formalin-fixed, paraffin-embedded tissues: Proteinase K, Pepsin, Pronase, Trypsin or Proteolytic Enzyme. As an alternative to enzyme pretreatment, heat-induced epitope retrieval may be used. For greater adherence of tissue sections to glass slides, the use of Silanized Slides is recommended. |
| Storage | |
Store between 2-8°C. |
| References | |
Tseng SCG, Jarvinen MJ, Nelson WG, Huang JW, Woodcock-Mitchell J, Sun TTl. Correlation of specific keratins with different types of epithelial differentiation: Monoclonal antibody studies. Cell 30:361, 1982. Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol 95:580, 1982. Moll R, Franke WW, Schiller Dl. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11, 1982. |
| Warranty/Conditions | |
Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | |
5/9/2008 |