血小板反应蛋白2(THBS2)重组蛋白

YB822Ra012
Recombinant Thrombospondin 2 (THBS2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 血小板反应蛋白2(THBS2)重组蛋白9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Asp968~Ile1172 (Accession # P35442),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.7
 血小板反应蛋白2(THBS2)重组蛋白Predicted Molecular Mass: 27.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 血小板反应蛋白2(THBS2)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSS HHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - DPK GTTQIDPNWV
IRHQGKELVQ TANSDPGIAV GFDEFGSVDF SGTFYVNTDR DDDYAGFVFG YQSSSRFYVV
MWKQVTQTYW EDQPT RAYGY SGVS LKVV NS TTGTGE H LR N ALWH TG NTPG
QVRTLWHDPR NIGWKDYTAY RWHLTHRPKT GYIRVLVHEG KQVMADSGPI YDQTYAGGRL
GLFVFSQEMV YFSDLKYECR DI