EndoFree Plasmid Maxiprep Kit，质粒提取试剂盒，中国库存，Biotool美国品牌。
产品名称： EndoFree Plasmid Maxiprep Kit，质粒提取试剂盒，中国库存，Biotool美国品牌。
英文名称： EndoFree Plasmid Maxiprep Kit
产品编号： B22216
产品价格： 0
产品产地： 美国
品牌商标： Biotool
更新时间： null
使用范围： null
EndoFree Plasmid Maxiprep Kit - Syringe Format
Advantages
40 minutes per run
Protocol contains only a final elution centrifugation using a 2 ml EP tube
< 0.3 EU (Endotoxin) per μg of plasmid DNA
>500 μg plasmid yield from 200 ml LB culture
No chaotropic salts present in buffer
Maxiprep Flow Chart
| 1. Lysis and neutralization | 2. Clear Lysate through a syringe filter | 3. Bind DNA to the DNA column | 4. Wash the column by a plunger | 5. Detach the column and insert to a 2 mL tube. Spin the column to dry and elute the DNA. |
Description
| Company | LB Culture | Total Plasmid | 260/280 | 260/230 | Endotoxin | Electrophoresis |
|---|---|---|---|---|---|---|
| Biotool | 150 ml | 461 μg | 1.82 | 2.01 | < 0.1 EU /μg |  |
| 200 ml | 556 μg | 1.83 | 2.11 | 0.265 EU /μg | ||
| 250 ml | 737 μg | 1.83 | 1.95 | 0.260 EU /μg | ||
| Invitrogen | 250 ml | 769 μg | 1.92 | 2.12 | 0.314 EU /μg | |
| QIAGEN | 250 ml | 609 μg | 1.83 | 2.10 | 0.262 EU /μg |
The purified endotoxin-free DNA is ready for high performance assays, such as transfection of endotoxin-sensitive cell lines and primary cultured cells or microinjection.
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