Product Description

Fibroblast Growth Supplement serum-free (FGS-2) is a medium supplement designed for the optimal growth of normal human fibroblasts in vitro. It is a sterile, concentrated (100X) solution which contains growth factors, hormones, and proteins necessary for the culture of normal human fibroblasts. The supplement is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced growth environment that maximally promotes the growth of normal human fibroblasts in vitro. The supplement is designed as an additive for Fibroblast Medium-serum free (FM-sf, Cat. No. 2311) and should be used in conjunction with that medium.

Components

FGS-2 is packaged in the quantity of supplement suited for a 500 ml bottle of fibroblast medium-serum free. When a 500 ml bottle of fibroblast medium-serum free is supplemented with FGS, the final concentrations of the supplement components per milliliter will be BSA 10 μg, apo-transferrin 10 μg, insulin 5 mg, EGF 5 ng, FGF-2 5 ng, IGF-1 0.1ng, aminobenzoic acid 0.1nM, arachidonic acid 0.12nM, linoleic acid 1nM, heparin 0.1U, and hydrocortisone 1 mg.

Product Use

FGS is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the FGS-2 at -20oC before adding to fibroblast medium.

Shipping

Dry ice.

Prepare for use

Thaw FGS-2 at 37oC. Gently tilt the FGS-2 tube several times during thawing to help the contents dissolve. Make sure the contents of the supplement are completely dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add FGS-2 and other component (P/S solution) into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of fibroblast medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37oC. When stored in the dark at 4oC, the reconstituted medium is stable for one month.