CARD4,凋亡加强结构域蛋白4抗体

产品编号HZ-7085R
英文名称CARD4
中文名称凋亡加强结构域蛋白4抗体
别 名CARD 4; CARD4; Caspase recruitment domain 4; Caspase recruitment domain containing protein 4; Caspase recruitment domain family member 4; Caspase recruitment domain protein 4; Caspase recruitment domain-containing protein 4; CLR 7.1; CLR7.1; NLR family CARD domain containing 1; NLRC 1; NLRC1; NOD 1; Nod1; NOD1 protein; NOD1_HUMAN; Nucleotide binding oligomerization domain containing 1; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1; Nucleotide-binding oligomerization domain-containing protein 1; Protein Nod1.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
CARD4,凋亡加强结构域蛋白4抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量108kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CARD4
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CARD4,凋亡加强结构域蛋白4抗体PubMedPubMed
产品介绍background:
Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB.

Subunit:
Homodimer. Self-associates. Binds to caspase-9 and RIPK2 by CARD-CARD interaction. Interacts with ARHGEF2.

Subcellular Location:
Cytoplasm. Basolateral cell membrane. Recruited to the basolateral membrane compartment in polarized epithelial cells.

Tissue Specificity:
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon.

Similarity:
Contains 1 CARD domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.

CARD4,凋亡加强结构域蛋白4抗体Database links:
Entrez Gene: 10392 Human
Entrez Gene: 107607 Mouse
Entrez Gene: 500133 Rat
Omim: 605980 Human
SwissProt: Q9Y239 Human
SwissProt: Q8BHB0 Mouse
Unigene: 405153 Human
Unigene: 28498 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CARD4 Polyclonal Antibody, Unconjugated(HZ-7085R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining