碘甲腺原氨酸脱碘酶Ⅲ(DIO3)重组蛋白

YB989Ra011
Recombinant Deiodinase, Iodothyronine, Type III (DIO3)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
碘甲腺原氨酸脱碘酶Ⅲ(DIO3)重组蛋白[ PROPERTIES ]
Residues: Arg11~Arg277 (Accession # Q91ZI8),
with two N-terminal Tags, His-tag and MBP-tag.
Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type II membrane protein. Endosome
membrane . Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
碘甲腺原氨酸脱碘酶Ⅲ(DIO3)重组蛋白pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 77.0kDa
Accurate Molecular Mass: 55kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
碘甲腺原氨酸脱碘酶Ⅲ(DIO3)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and MBP-tag, its
sequence is listed below.
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI
IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK
DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK
DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK
VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL
GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE
ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ
GPLGSEF- RLCAQTASCL VLFPRFLGTA FMLWLLDFLC IRKHFLRRRH PDHPEPEVEL
NS E GE E MP P D DP PI CV S D D N R L C T LA S LK A VW H G Q K L D F F KQA HE G GPA P
NSEVVRPDGF QSQRILDYAQ GTRPLVLNFG SCTUPPFMAR MSAFQRLVTK YQRDVDFLII
YIEEAHPSDG WVTTDSPYVI PQHRSLEDRV SAARVLQQGA PGCALVLDTM ANSSSSAYGA
YFERLYVIQS GTIMYQGGRG PDGYQVSELR TWLERYDEQL HGTRPHR