小鼠磷酸肌醇-3-激酶2α肽(PIK3C2a)多克隆抗体
Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)
[PRODUCT INFORMATION]
Immunogen: PIK3C2a, Mouse
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant PIK3C2a (Lys349~Pro611) expressed in E.coli. 
Sequence: The target protein is fused with two N-terminal Tags, His-tag andGST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-KA QGQVSQKDPNGTSSLPTGSS LLQEFEVQND EVAAFCQSIM KLKTKFPYTD HCTNPGYLLS PVTVQRNMCGENASVKVSIE IEGLQLPVTF TCDVSSTVEI IIMQALCWVH DDLNQVDVGS YILKVCGQEEVLQNNHCLGS HEHIQNCRKW DTEIKLQLLT LSAMCQNLAR TAEDDEAPVD LNKYLYQIEKPYKEVMTRHP VEELLDSYHY QVELALQTEN QHRAVDQVIK AVRKICSALD GVETPSVTEAVKKLKRAVNL P
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against PIK3C2a. It has been
selected for its ability to recognize PIK3C2a in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[IMAGES ]

[CONTENTS ] 
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN₃, 50%glycerol.
[ QUALITY CONTROL ] 
Content:The quality control contains recombinant PIK3C2a (Lys349~Pro611)disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN₃ 0.02%
[STORAGE ] 
Store at 4 °C for frequent use. Stored at -20°C to -80 °C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。