亲核素β(KPNβ)重组蛋白

YB752Hu012
Recombinant Karyopherin Beta (KPNb1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
亲核素β(KPNβ)重组蛋白[ PROPERTIES ]
Residues: Ala21~Glu441
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q14974
Host: E. coli
Subcellular Location: Cytoplasm, Nucleus
envelope.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
亲核素β(KPNβ)重组蛋白(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 4.7
Predicted Molecular Mass: 50.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
亲核素β(KPNβ)重组蛋白precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
AQKFLERAAV ENLPTFLVEL SRVLANPGNS QVARVAAGLQ IKNSLTSKDP DIKAQYQQRW
LAIDANARRE VKNYVLQTLG TETYRPSSAS QCVAGIACAE IPVNQWPELI PQLVANVTNP
NSTEHMKEST LEAIGYICQD IDPEQLQDKS NEILTAIIQG MRKEEPSNNV KLAATNALLN
SLEFTKANFD KESERHFIMQ VVCEATQCPD TRVRVAALQN LVKIMSLYYQ YMETYMGPAL
FAITIEAMKS DIDEVALQGI EFWSNVCDEE MDLAIEASEA AEQGRPPEHT SKFYAKGALQ
YLVPILTQTL TKQDENDDDD DWNPCKAAGV CLMLLATCCE DDIVPHVLPF IKEHIKNPDW
RYRDAAVMAF GCILEGPEPS QLKPLVIQAM PTLIELMKDP SVVVRDTAAW TVGRICELLP E