ScienCells GeneQuery™ human astrocyte cell biology qPCR array kit (GQH-AST) is designed to facilitate gene expression profiling of key genes involved in (i) human astrocyte development and maintenance; (ii) genes that are much up-regulated in astrocytes; and (iii) related signaling pathways, such as Notch, TGFB, JAK/STAT, and MAPK pathways. 88 genes are selected in this kit based on public database and literature research.      

GeneQuery™ PCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target genes cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg²⁺, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 

GeneQuery™ Human Astrocyte Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)

* gene selection may be updated based on new research and development