含Src同源物2域适配蛋白B(SHB)重组蛋白

YB223Mu012
Src Homology 2 Domain Containing Adapter Protein B (SHB)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
含Src同源物2域适配蛋白B(SHB)重组蛋白[ PROPERTIES ]
Residues: Ala252~Arg507 (Accession # Q15464),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cytoplasm. Cell
membrane; Peripheral membrane protein;
Cytoplasmic side.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
含Src同源物2域适配蛋白B(SHB)重组蛋白pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 9.0
Predicted Molecular Mass: 32.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
含Src同源物2域适配蛋白B(SHB)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- AKNDLKSKA GKGESAGYME
PYEAQRIMTE FQRQESVRSQ HKGIQLYDTP YEPEGQSVDS DSESTVSPRL RESKLPQDDD
RPA DEY DQPW EW NRVTI PA L AAQ F NG NEK R QSSPS PS R DR RRQ L RAP GG G
FKPIKHGSPE FCGILGERVD PAVPLEKQIW YHGAISRGDA ENLLRLCKEC SYLVRNSQTS
KHDYSLSLRS NQGFMHMKLA KTKEKYVLGQ NSPPFDSVPE VIHYYTTRKL PIKGAEHLSL
LYPVAVR