UBE2S抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
UBE2S抗体

UBE2S抗体

商家询价

产品名称： UBE2S抗体

英文名称： UBE2SAntibody

产品编号： XY 14115-1

产品价格： null

产品产地： 中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
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 UBE2S抗体Applications

In-house applications ELISA, WB, IHC, FC, IP     Application not tested? Please see our Guarantee.
Species specificity Human,Mouse,Rat; other species not tested.
Images
Prev12NextView more validation data
  • 14115-1-AP;MDA-MB-453s cellsMDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14115-1-AP(UBE2S antibody) at dilution of 1:500
  • 14115-1-AP;human breast cancerImmunohistochemical of paraffin-embedded human breast cancer using 14115-1-AP(UBE2S antibody) at dilution of 1:50 (under 10x lens)
  • 14115-1-AP;human breast cancerImmunohistochemical of paraffin-embedded human breast cancer using 14115-1-AP(UBE2S antibody) at dilution of 1:50 (under 40x lens)
  • 14115-1-AP;A2780 cellsIP Result of anti-UBE2S (IP:14115-1-AP, 4ug; Detection:14115-1-AP 1:1000) with A2780 cells lysate 960ug.
  • 14115-1-AP;Jurkat cells1X10^6 Jurkat cells were stained with 0.5ug UBE2S antibody (14115-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Positive WB detected in MDA-MB-453s cells, A2780 cells, human liver tissue, MCF7 cells, mouse liver tissue, mouse ovary tissue
Positive IP detected in A2780 cells
Positive IHC detected in Human breast cancer tissue, human ovary tumor tissue, human skin tissue
Positive FC detected in Jurkat cells
Recommended dilution WB : 1:200-1:2000
IP : 1:500-1:5000
IHC : 1:20-1:200
 UBE2S抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information UBE2S抗体
Immunogen UBE2S fusion protein ag5264 Full name Ubiquitin-conjugating enzyme E2S
Calculated molecular
weight		 222aa,24 kDa Observed molecular 
weight		 28 kDa
GenBank accession 
number		 BC066948 Gene ID (NCBI) 27338
Gene symbol UBE2S Synonyms E2 EPF, E2EPF, EPF5, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S
Background

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549). UBE2S抗体

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