Bak,Bcl-2同源拮抗剂抗体

产品编号HZ-1284R
英文名称Bak
中文名称Bcl-2同源拮抗剂抗体
别 名Apoptosis Regulator Bak; BAK 1; BAK; BAK like; Bak NT; BAK1; Bcl 2 homologous antagonist/killer; Bcl 2 like 7 protein; Bcl2 homologous antagonist killer; Bcl2 like 7 Protein; BCL2-antagonist/killer 1; BCL2L7; CDN 1; CDN1; Cell death inhibitor 1; MGC117255; MGC3887; NBak; Pro apoptotic protein BAK; BAX_MOUSE.
说 明 书0.1ml 0.2ml
研究领域免疫学 细胞凋亡
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
Bak,Bcl-2同源拮抗剂抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量23kDa
细胞定位细胞浆 细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from mouse Bak
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Bak,Bcl-2同源拮抗剂抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq, Jul 2008]

Function:
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.

Subunit:
Belongs to the Bcl-2 family.

Subcellular Location:
Mitochondrion membrane.

Tissue Specificity:
Expressed in a wide variety of tissues.

Similarity:
Belongs to the Bcl-2 family.

Gene ID:
578

Bak,Bcl-2同源拮抗剂抗体Database links:
Entrez Gene: 578 Human
Entrez Gene: 12018 Mouse
Omim: 600516 Human
SwissProt: Q16611 Human
SwissProt: Q6I9T6 Human
SwissProt: O08734 Mouse
Unigene: 485139 Human
Unigene: 2443 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Bak属于bcl-2基因家族中的成员，具有抑制凋亡的作用。BAK基因是新发现的细胞凋亡促进因子，BAK含BH1和BH2结构域，其功能类似于BAX；拮抗BCL2、BCL-XL的抗凋亡效应。

产品图片

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Bak Polyclonal Antibody, Unconjugated(HZ-1284R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining