RAD50,DNA修复蛋白Rad50抗体

产品编号HZ-11230R
英文名称RAD50
中文名称DNA修复蛋白Rad50抗体
别 名DNA repair protein RAD50; hRad50; hsRAD50; Mrell; NBSLD; Rad 50; RAD50 2; rad50; RAD50 homolog (S cerevisiae); RAD50 homolog; RAD50 PEN; RAD50_HUMAN; Rad50l; Truncated RAD50 protein.
说 明 书0.1ml 0.2ml
研究领域染色质和核信号 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,
RAD50,DNA修复蛋白Rad50抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量154kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human RAD50 (817-872aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
RAD50,DNA修复蛋白Rad50抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.

Function:
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.

RAD50,DNA修复蛋白Rad50抗体Subunit:
Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis.

Subcellular Location:
Nucleus. Chromosome telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.

Tissue Specificity:
Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR

DISEASE:
Defects in RAD50 are the cause of Nijmegen breakage syndrome-like disorder (NBSLD) [MIM:613078]; also called NBS-like disorder or RAD50 deficiency. NBSLD is a disorder similar to Nijmegen breakage syndrome and characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, short stature and bird-like face. Immunodeficiency is absent.

Similarity:
Belongs to the SMC family. RAD50 subfamily.
Contains 1 zinc-hook domain.

Gene ID:
10111

Database links:
Entrez Gene: 10111 Human
Entrez Gene: 19360 Mouse
Entrez Gene: 64012 Rat
Omim: 604040 Human
SwissProt: Q92878 Human
SwissProt: P70388 Mouse
SwissProt: Q9JIL8 Rat
Unigene: 633509 Human
Unigene: 4888 Mouse
Unigene: 51136 Rat

RAD50,DNA修复蛋白Rad50抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.