ATP结合盒转运蛋白B5(ABCB5)重组蛋白

YB635Hu016
Recombinant ATP Binding Cassette Transporter B5 (ABCB5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
ATP结合盒转运蛋白B5(ABCB5)重组蛋白 PROPERTIES ]
Residues: Leu570~Ala808
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q2M3G0
Host: E. coli
Subcellular Location: Cell membrane; Multi-pass
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(ATP结合盒转运蛋白B5(ABCB5)重组蛋白determined by the LAL method).

Formulation: Supplied as lyophilized form in 100mM
NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA,
1mM DTT, 0.01% sarcosyl, 5% trehalose, and
preservative.
Predicted isoelectric point: 8.4
Predicted Molecular Mass: 30.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
ATP结合盒转运蛋白B5(ABCB5)重组蛋白L EFREVSFFYP CRPDVFILRG LSLSIERGKT VAFVGSSGCG KSTSVQLLQR LYDPVQGQVL
FDGVDAKELN VQWLRSQIAI VPQEPVLFNC SIAENIAYGD NSRVVPLDEI KEAANAANIH
SFIEGLPEKY NTQVGLKGAQ LSGGQKQRLA IARALLQKPK ILLLDEATSA LDNDSEKVVQ
HALDKARTGR TCLVVTHRLS AIQNADLIVV LHNGKIKEQG THQELLRNRD IYFKLVNA
[ REFERENCES ]
1. Frank N.Y., et al. (2003) J. Biol. Chem. 278:47156-47165.
2. Chen K.G., et al. (2005) Pigment Cell Res. 18:102-112.
3. Wilson B.J., et al. (2011) Cancer Res. 71:5307-5316.
4. Kawanobe T., et al. (2012) Biochem. Biophys. Res. Commun. 418:736-741.