2x SYBR Green qPCR Master Mix

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Advantages

Accurate Detection: High specific and sensitive quantitation even for low copy targets or a wide range of template samples
High Stability: Hot-start enzyme activation and convenient room-temperature reaction setup makes it suitable for automation and high throughput detection
Robustness and Versatility: High-efficiency, compatible with both fast and standard qPCR cycling methods
Cost Effective: Offered at much discounted price compared to market price

Description

Biotool™ 2x SYBR Green qPCR master mix utilizes a special performance-enhanced Taq DNA polymerase protected via a hot-start activation technique, and optimized qPCR buffer system to perform SYBR Green I based quantitative PCR. The mix, prepared at 2x reaction concentration, can be directly used for robust and low-template quantitative PCR with high sensitivity, specificity and reliability.

Applications

Dye-based quantitative PCR detection
Nucleic acid amplification and expression profiling
Quantitative genotyping studies
Genomics-related applications

Components

Component	Cat #: B21201	Cat #: B21202	Cat #: B21203
Biotool™ 2x SYBR Green qPCR Master Mix[a]	1.25 ml (for 50 reactions with 50 μl/rxn)	5 ml (for 200 reactions with 50 μl/rxn)	25 ml (for 1000 reactions with 50 μl/rxn)
50x ROX Dye (low conc.)	50 μl	200 μl	1000 μl
50x ROX Dye (high conc.)	50 μl	200 μl	1000 μl
[a] Contains hot-start DNA Polymerase, dNTPs, Mg2+, and SYBR Green I dye

相关产品：
1，Biotool SYBR Green One Step qRT-PCR Kit
2，2x SYBR Green qPCR Master Mix (Low ROX)
3，2x SYBR Green qPCR Master Mix (High ROX)
4，2x SYBR Green Fast qPCR Master Mix 
5，2x Probe qPCR Master Mix
 
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