Sulfo-NHS-SS-Biotin

中文名称：Sulfo-NHS-SS-Biotin
英文名称：sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate
产品规格：50mg
分子量：606.7
储存条件：冷冻（-20℃）

概述

Sulfo-NHS-SS-Biotin(sulfosuccinimidyl-2-[biotinamido]ethyl-1,3-dithiopropionate)is a thiol-cleavable amine-reactive
biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding.

应用

Thisreagent is water-soluble(to approximately 10mM)enabling biotinylation in the absence of organic solvents such as DMSO
or DMF.Water-solubility is advantageous for applications that cannot tolerate solvents or when inclusion of solvents
complicates the protocol.
The N-hydroxysulfosuccinimide(NHS)ester group on this reagent reacts with the ε-amine of lysine residues to produce a
stable product.Although α-amine groups present on the N-termini of peptides react with NHS esters,α-amines on proteins
are seldom accessible for conjugation.NHS esters react with primary amines in the deprotonated form and,therefore,the
reaction requires neutral to basic pH values to proceed.Primary amines react with NHS esters by nucleophilic attack and
Nhydroxysulfosuccinimideis released as a byproduct.Hydrolysis of the NHS-ester competes with the reaction in aqueous
solution.The rate of hydrolysis increases with increasing pH and occurs more readily in dilute protein solutions.

组份

50mg/bottle

技术规格
 

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