GALR2,甘丙肽受体2抗体

产品编号HZ-11527R
英文名称GALR2
中文名称甘丙肽受体2抗体
别 名GAL2-R; GAL2R; Galanin receptor 2; Galanin receptor type 2; GALNR2; GALR-2; GALR2; GALR2_HUMAN; MGC125983; MGC125984.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 神经生物学 信号转导 通道蛋白 细胞膜受体 G蛋白偶联受体 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
GALR2,甘丙肽受体2抗体产品应用WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-200 ICC=1:10-100 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量42kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human GALR2 (51-150aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
GALR2,甘丙肽受体2抗体PubMedPubMed
产品介绍background:
The 387 amino acid protein galanin receptor-2 (GALR2) protein belongs to a family of G protein-coupled receptors that specifically bind galanin, a neuropeptide distributed throughout the central and peripheral nervous system, the pituitary gland, the gastrointestinal tract and in the endocrine and exocrine pancreas (1-3). GALR2 mRNA is abundant in parvocellular paraventricular nuclei, but in contrast to GALR1, is not expressed in magnocellular neurons or in supraoptic nuclei. Like GALR1 mRNA, GALR2 mRNA is expressed in the POMC neurons, dorsomedial nucleus, arcuate nucleus and in restricted peripheral tissue, with highest mRNA levels in human small intestine (2-5). Galanin-like peptide (GALP) is a putative endogenous ligand for GALR2 (2). Binding of GALP to GALR2 results in increased GTPgS binding to the membrane-bound GALR2 (6). GALR2 is therefore a receptor that mediates important functions of galanin in the hypothalamic-pituitary axis, plays a role in hippocampal and cerebellar function (7) and mediates jejunal contraction (8).

Function:
Receptor for the hormone galanin and for GALP. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibit adenylyl cyclase.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Expressed abundantly within the central nervous system in both hypothalamus and hippocampus. In peripheral tissues, the strongest expression was observed in heart, kidney, liver, and small intestine.

GALR2,甘丙肽受体2抗体Similarity:
Belongs to the G-protein coupled receptor 1 family.

Gene ID:
8811

Database links:
UniProtKB/Swiss-Prot: O43603.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GALR2 Polyclonal Antibody, Unconjugated(HZ-11527R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining